Revisiting the specificity of the MHC class II transactivator CIITA in vivo.

Détails

ID Serval
serval:BIB_0DCB58DD80FC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Revisiting the specificity of the MHC class II transactivator CIITA in vivo.
Périodique
European Journal of Immunology
Auteur(s)
Otten L.A., Leibundgut-Landmann S., Huarte J., Kos-Braun I.C., Lavanchy C., Barras E., Borisch B., Steimle V., Acha-Orbea H., Reith W.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
2006
Volume
36
Numéro
6
Pages
1548-1558
Langue
anglais
Résumé
CIITA is a master regulatory factor for the expression of MHC class II (MHC-II) and accessory genes involved in Ag presentation. It has recently been suggested that CIITA also regulates numerous other genes having diverse functions within and outside the immune system. To determine whether these genes are indeed relevant targets of CIITA in vivo, we studied their expression in CIITA-transgenic and CIITA-deficient mice. In contrast to the decisive control of MHC-II and related genes by CIITA, nine putative non-MHC target genes (Eif3s2, Kpna6, Tap1, Yars, Col1a2, Ctse, Ptprr, Tnfsf6 and Plxna1) were found to be CIITA independent in all cell types examined. Two other target genes, encoding IL-4 and IFN-gamma, were indeed found to be up- and down-regulated, respectively, in CIITA-transgenic CD4(+) T cells. However, there was no correlation between MHC-II expression and this Th2 bias at the level of individual transgenic T cells, indicating an indirect control by CIITA. These results show that MHC-II-restricted Ag presentation, and its indirect influences on T cells, remains the only pathway under direct control by CIITA in vivo. They also imply that precisely regulated MHC-II expression is essential for maintaining a proper Th1-Th2 balance.
Mots-clé
Animals, Female, Flow Cytometry, Gene Expression Regulation/genetics, Gene Expression Regulation/immunology, Histocompatibility Antigens Class II/biosynthesis, Histocompatibility Antigens Class II/genetics, Interferon-gamma/biosynthesis, Interferon-gamma/genetics, Interleukin-4/biosynthesis, Interleukin-4/genetics, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Knockout, Mice, Transgenic, Nuclear Proteins/biosynthesis, Nuclear Proteins/genetics, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Substrate Specificity, Th1 Cells/immunology, Th2 Cells/immunology, Trans-Activators/biosynthesis, Trans-Activators/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:48
Dernière modification de la notice
03/03/2018 13:40
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