Drosophila Myc interacts with host cell factor (dHCF) to activate transcription and control growth.

Détails

ID Serval
serval:BIB_0D963B2F4F6E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Drosophila Myc interacts with host cell factor (dHCF) to activate transcription and control growth.
Périodique
Journal of Biological Chemistry
Auteur(s)
Furrer M., Balbi M., Albarca-Aguilera M., Gallant M., Herr W., Gallant P.
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
285
Numéro
51
Pages
39623-39636
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
The Myc proto-oncoproteins are transcription factors that recognize numerous target genes through hexameric DNA sequences called E-boxes. The mechanism by which they then activate the expression of these targets is still under debate. Here, we use an RNAi screen in Drosophila S2 cells to identify Drosophila host cell factor (dHCF) as a novel co-factor for Myc that is functionally required for the activation of a Myc-dependent reporter construct. dHCF is also essential for the full activation of endogenous Myc target genes in S2 cells, and for the ability of Myc to promote growth in vivo. Myc and dHCF physically interact, and they colocalize on common target genes. Furthermore, down-regulation of dHCF-associated histone acetyltransferase and histone methyltransferase complexes in vivo interferes with the Myc biological activities. We therefore propose that dHCF recruits such chromatin-modifying complexes and thereby contributes to the expression of Myc targets and hence to the execution of Myc biological activities.
Mots-clé
Animals, Cell Line, Cell Proliferation, Chromatin/genetics, Chromatin/metabolism, DNA-Binding Proteins/immunology, DNA-Binding Proteins/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/immunology, Drosophila melanogaster, Gene Expression Regulation/physiology, Histone Acetyltransferases/genetics, Histone Acetyltransferases/metabolism, Histone-Lysine N-Methyltransferase/genetics, Histone-Lysine N-Methyltransferase/metabolism, Multienzyme Complexes/genetics, Multienzyme Complexes/metabolism, Transcription Factors/immunology, Transcription Factors/metabolism, Transcription, Genetic/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2010 8:50
Dernière modification de la notice
08/05/2019 14:23
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