Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications.

Détails

ID Serval
serval:BIB_0D913C03B38A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications.
Périodique
Genome Research
Auteur(s)
Hilson P., Allemeersch J., Altmann T., Aubourg S., Avon A., Beynon J., Bhalerao R.P., Bitton F., Caboche M., Cannoot B., Chardakov V., Cognet-Holliger C., Colot V., Crowe M., Darimont C., Durinck S., Eickhoff H., de Longevialle A.F., Farmer E.E., Grant M., Kuiper M.T., Lehrach H., Léon C., Leyva A., Lundeberg J., Lurin C., Moreau Y., Nietfeld W., Paz-Ares J., Reymond P., Rouzé P., Sandberg G., Segura M.D., Serizet C., Tabrett A., Taconnat L., Thareau V., Van Hummelen P., Vercruysse S., Vuylsteke M., Weingartner M., Weisbeek P.J., Wirta V., Wittink F.R., Zabeau M., Small I.
ISSN
1088-9051 (Print)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
2004
Volume
14
Numéro
10B
Pages
2176-2189
Langue
anglais
Résumé
Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
Mots-clé
Arabidopsis/genetics, Arabidopsis/growth &amp, development, Arabidopsis Proteins/genetics, DNA Primers/chemistry, DNA Primers/genetics, DNA, Plant/genetics, Databases, Genetic, Expressed Sequence Tags, Gene Expression Profiling, Gene Expression Regulation, Plant, Genome, Plant, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, RNA Interference, RNA, Messenger/genetics, RNA, Plant/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 21:05
Dernière modification de la notice
08/05/2019 14:23
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