'Lipidic particle' systems as visualized by thin-section electron microscopy

Détails

ID Serval
serval:BIB_0D3D9F3EED66
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
'Lipidic particle' systems as visualized by thin-section electron microscopy
Périodique
Biochimica et Biophysica Acta - Biomembranes
Auteur(s)
Verkleija A.J., Humbel B., Studerb D., Müller M.
ISSN
0005-2736
Statut éditorial
Publié
Date de publication
1985
Volume
812
Numéro
2
Pages
591-594
Langue
anglais
Résumé
Lipidic phases, containing 'lipidic particles' (cardiolipin/egg lecithin in the presence of Ca2+ and egg lecithin/dioleoylphosphatidylcholine/cholesterol) have been investigated with an alternative thin-section method, using rapid cryofixation and freeze substitution according to Müller et al. (Müller, M., Marti, T. and Kriz, S. (1980) in Proc. 7th Eur. Congr. on Electron Microscopy (Brederoo, P. and De Priester, W., eds.), pp. 720-721) in combination with low temperature embedding in Lowicryl HM20 according to Humbel et al. (Humbel, B. and Müller M. (1984) in Proc. 8th Eur. Congr. on Electron Microscopy (Csanady, P., Röhlich, P.; Szabo D., eds.), pp. 1789-1798). With this method one can visualize a honeycomb structure with local fusion (joining) points between bilayers which is compatible with the structures hypothesized for lipidic particle-containing systems.
Mots-clé
Lipidic particle, Membrane fusion, Phospholipid bilayer, Cryofixation, Electron microscopy
Web of science
Création de la notice
18/10/2012 14:00
Dernière modification de la notice
03/03/2018 13:39
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