Evidence of gestational heroin or nicotine exposure by analysis of fetal hair.

Détails

ID Serval
serval:BIB_0C253642142A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evidence of gestational heroin or nicotine exposure by analysis of fetal hair.
Périodique
Forensic Science International
Auteur(s)
Kintz P., Mangin P.
ISSN
0379-0738[print], 0379-0738[linking]
Statut éditorial
Publié
Date de publication
1993
Volume
63
Numéro
1-3
Pages
99-104
Langue
anglais
Résumé
Failure to identify tobacco- or heroin-exposed neonates is extensive owing to the limitations of current methods used to verify maternal drug use. Maternal self-reported drug history has been shown to be unreliable: many women who deny use during pregnancy exhibit drug metabolites in their urine. Maternal systematic urinalysis is hampered by the short elimination half-life of the drugs. This test is not suitable for validation of survey data since the quantification of drugs in urine only reflects exposure during the preceding 1-3 days and does not necessarily indicate the frequency in subjects who might deliberately abstain for several days before biomedical screenings. The same disadvantages are noted with the analysis of babies' meconium, or the amniotic fluids which are only a qualitative test at the moment of delivery. Hair analysis remedies the disadvantages of these currently available methods by exhibiting a wide window of detection and may provide information concerning the severity of gestational exposure. Hair samples were collected at time of delivery from 40 and 9 neonates whose mothers were known, by self-report, to be smokers and heroin users during the prenatal period, respectively. Hair was decontaminated in dichloromethane (37 degrees C, 15 min) and homogenizated in NaOH (1 M, 10 min, 90 degrees C). Nicotine and opiates were extracted in diethylether at pH 14, and in chloroform/isopropanol/n-heptane (50:17:33 v/v) at pH 9.2, respectively. After separation on a BP-5 capillary column, drugs were identified and quantified by GC/MS using selected ion monitoring. The ranges of measured concentrations were 0.15-11.80 and 0.61-3.47 ng/mg for nicotine and morphine, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Female, Gas Chromatography-Mass Spectrometry, Hair/chemistry, Heroin/analysis, Humans, Infant, Newborn, Maternal-Fetal Exchange, Morphine/analysis, Nicotine/analysis, Pregnancy, Smoking, Street Drugs/analysis, Substance Abuse Detection/methods
Pubmed
Création de la notice
18/10/2010 15:07
Dernière modification de la notice
03/03/2018 13:37
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