Article: article from journal or magazin.
Case report (case report): feedback on an observation with a short commentary.
Histological, immunohistochemical, ultrastructural and biochemical study of human gastric composite tumor: expression of the serotonin-2B receptor by the neuroendocrine component.
Publication types: Case Reports ; Journal ArticlePublication Status: ppublish
We report a case of a human gastric composite tumor occurring seven years after a partial gastrectomy for a low grade B cell MALT lymphoma. Histological examination of the tumor revealed two intimately intermingled components: 1. A moderately to poorly differentiated tubulo-acinar adenocarcinoma with signet-ring cells; and 2. Isolated or clustered small neuroendocrine cells without atypia expressing chromogranin A, somatostatin and/or glucagon, serotonin (5-HT) and, the 5-HT2B receptors. In addition to immunohistochemical detection, the presence of 5-HT2B receptors was shown pharmacologically through [125I]-DOI binding. Since 5-HT2B receptors have been demonstrated to have autocrine functions and, mitogenic and transforming properties, these results suggest a role of 5-HT in neuroendocrine malignant transformation. On the other hand, the expression of somatostatin and the detection by reverse transcriptase polymerase chain reaction (RT-PCR) of somatostatin receptor subtypes 2, 3, and 5, which have been shown to be involved in tumor regression, might account for the long evolution of this case (> 5 yr). This case illustrates the importance of local humoral modulation in tumor growth. Moreover, ultrastructural results favor a unique origin of the tumor cells from one amphicrine cell type.
Adenocarcinoma/chemistry, Adenocarcinoma/metabolism, Chromogranin A, Chromogranins/analysis, Chromogranins/metabolism, Cytoplasmic Granules/ultrastructure, Female, Glucagon/analysis, Glucagon/metabolism, Humans, Immunoenzyme Techniques, Middle Aged, Neoplasms, Second Primary/chemistry, Neoplasms, Second Primary/metabolism, Neuroendocrine Tumors/chemistry, Neuroendocrine Tumors/metabolism, RNA, Messenger/analysis, RNA, Messenger/metabolism, RNA, Neoplasm/analysis, Receptor, Serotonin, 5-HT2B, Receptors, Serotonin/analysis, Receptors, Serotonin/genetics, Receptors, Somatostatin/analysis, Receptors, Somatostatin/classification, Reverse Transcriptase Polymerase Chain Reaction, Serotonin/analysis, Serotonin/metabolism, Somatostatin/analysis, Somatostatin/classification, Stomach Neoplasms/chemistry, Stomach Neoplasms/metabolism
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