TRAIP is a regulator of the spindle assembly checkpoint.

Détails

Ressource 1Télécharger: 5_25335891_Postprint.pdf (6706.22 [Ko])
Etat: Serval
Version: Author's accepted manuscript
Document(s) secondaire(s)
Télécharger: 5149.full.pdf (9055.03 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_0B0118FCA445
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
TRAIP is a regulator of the spindle assembly checkpoint.
Périodique
Journal of Cell Science
Auteur(s)
Chapard C., Meraldi P., Gleich T., Bachmann D., Hohl D., Huber M.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
127
Numéro
24
Pages
5149-5156
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Accurate chromosome segregation during mitosis is temporally and spatially coordinated by fidelity-monitoring checkpoint systems. Deficiencies in these checkpoint systems can lead to chromosome segregation errors and aneuploidy, and promote tumorigenesis. Here, we report that the TRAF-interacting protein (TRAIP), a ubiquitously expressed nucleolar E3 ubiquitin ligase important for cellular proliferation, is localized close to mitotic chromosomes. Its knockdown in HeLa cells by RNA interference (RNAi) decreased the time of early mitosis progression from nuclear envelope breakdown (NEB) to anaphase onset and increased the percentages of chromosome alignment defects in metaphase and lagging chromosomes in anaphase compared with those of control cells. The decrease in progression time was corrected by the expression of wild-type but not a ubiquitin-ligase-deficient form of TRAIP. TRAIP-depleted cells bypassed taxol-induced mitotic arrest and displayed significantly reduced kinetochore levels of MAD2 (also known as MAD2L1) but not of other spindle checkpoint proteins in the presence of nocodazole. These results imply that TRAIP regulates the spindle assembly checkpoint, MAD2 abundance at kinetochores and the accurate cellular distribution of chromosomes. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control.
Pubmed
Web of science
Création de la notice
17/01/2015 12:05
Dernière modification de la notice
03/03/2018 13:35
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