Article: article from journal or magazin.
Capillary electrophoresis as a second dimension to isoelectric focusing for peptide separation.
Publication types: Comparative Study ; Journal Article Publication Status: ppublish
Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, beta-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of capillary electrophoresis as a second dimension to Off-Gel isoelectric focusing, the optimized two-dimensional platforms provide a degree of orthogonality comparable to state-of-the-art multidimensional liquid chromatography systems as well as a practical peak capacity above 700.
Animals, Chromatography, Liquid/methods, Cytochromes c/isolation & purification, Cytochromes c/metabolism, Electrophoresis, Capillary/methods, Isoelectric Focusing/methods, Lactoglobulins/isolation & purification, Lactoglobulins/metabolism, Myoglobin/isolation & purification, Myoglobin/metabolism, Peptide Fragments/isolation & purification, Peptide Fragments/metabolism, Serum Albumin, Bovine/isolation & purification, Serum Albumin, Bovine/metabolism, Trypsin/metabolism
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