Frequent coexpression of cytolytic activity and lymphokine production among human T lymphocytes. Production of B cell growth factor and interleukin 2 by T8+ and T4+ cytolytic clones

Details

Serval ID
serval:BIB_0AF0017443C8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Frequent coexpression of cytolytic activity and lymphokine production among human T lymphocytes. Production of B cell growth factor and interleukin 2 by T8+ and T4+ cytolytic clones
Journal
European Journal of Immunology
Author(s)
Mingari  M. C., Moretta  A., Maggi  E., Pantaleo  G., Gerosa  F., Romagnani  S., Moretta  L.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/1984
Volume
14
Number
11
Pages
1066-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
These studies were designed to determine the degree of overlapping between cytolytic function and lymphokine production among peripheral blood human T lymphocytes. T8+ and T4+ cells were obtained by sorting purified T cells using the fluorescence-activated cell sorter (FACS) and cloned at 0.25 cells/well in a microculture system that allows clonal growth of every T cell. 49/49 T8+ clones but only 5/102 T4+ clones displayed cytolytic activity, as assessed by a lectin-dependent assay. Cytolytic clones were further analyzed for their ability to release interleukin 2 (IL2) and B cell growth factor(s) (BCGF) upon 24 h stimulation with phytohemagglutinin. Fifteen percent and 18% of T8+ cytolytic clones were producing IL2 and BCGF, respectively; in addition 3 out of 5 T4+ cytolytic clones released both lymphokines. Although the majority of cytolytic clones producing IL2 were also producing BCGF, 5 clones released either BCGF or IL2. The present studies indicate that the coexpression of cytolytic and helper/inducer functions is relatively common among peripheral blood T lymphocytes, irrespective of their T8+ or T4+ phenotype.
Keywords
B-Lymphocytes/*immunology Cells, Cultured Clone Cells *Cytotoxicity, Immunologic Flow Cytometry Growth Substances/*biosynthesis Humans Interleukin-2/*biosynthesis Interleukin-4 Lymphokines/*biosynthesis T-Lymphocytes/cytology/*immunology
Pubmed
Web of science
Create date
25/01/2008 15:13
Last modification date
20/08/2019 12:32
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