Gene duplication and neofunctionalization: POLR3G and POLR3GL.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_0AEC073C49B3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Gene duplication and neofunctionalization: POLR3G and POLR3GL.
Périodique
Genome Research
Auteur(s)
Renaud M., Praz V., Vieu E., Florens L., Washburn M.P., L'hôte P., Hernandez N.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
2014
Volume
24
Numéro
1
Pages
37-51
Langue
anglais
Résumé
RNA polymerase III (Pol III) occurs in two versions, one containing the POLR3G subunit and the other the closely related POLR3GL subunit. It is not clear whether these two Pol III forms have the same function, in particular whether they recognize the same target genes. We show that the POLR3G and POLR3GL genes arose from a DNA-based gene duplication, probably in a common ancestor of vertebrates. POLR3G- as well as POLR3GL-containing Pol III are present in cultured cell lines and in normal mouse liver, although the relative amounts of the two forms vary, with the POLR3G-containing Pol III relatively more abundant in dividing cells. Genome-wide chromatin immunoprecipitations followed by high-throughput sequencing (ChIP-seq) reveal that both forms of Pol III occupy the same target genes, in very constant proportions within one cell line, suggesting that the two forms of Pol III have a similar function with regard to specificity for target genes. In contrast, the POLR3G promoter-not the POLR3GL promoter-binds the transcription factor MYC, as do all other promoters of genes encoding Pol III subunits. Thus, the POLR3G/POLR3GL duplication did not lead to neo-functionalization of the gene product (at least with regard to target gene specificity) but rather to neo-functionalization of the transcription units, which acquired different mechanisms of regulation, thus likely affording greater regulation potential to the cell.
Pubmed
Web of science
Open Access
Oui
Création de la notice
23/01/2014 9:43
Dernière modification de la notice
20/08/2019 13:32
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