A method for anchoring round shaped cells for atomic force microscope imaging

Détails

ID Serval
serval:BIB_0ABD19909250
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A method for anchoring round shaped cells for atomic force microscope imaging
Périodique
Biophysical Journal
Auteur(s)
Kasas  S., Ikai  A.
ISSN
0006-3495
Statut éditorial
Publié
Date de publication
05/1995
Peer-reviewed
Oui
Volume
68
Numéro
5
Pages
1678-1680
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have to be strongly attached to the substrate, and imaging is restricted to cells having a flattened shape. Here we propose a simple and inexpensive solution to overcome these limitations. The method we propose is trapping living round shaped cells in a Millipore filter with a pore size comparable to the dimensions of the cell. The highest part of some of the blocked cells protrude through the holes of the filter and can this way be easily observed using the AFM without detachment.
Mots-clé
Micropore Filters Microscopy, Atomic Force/instrumentation/*methods Saccharomyces cerevisiae/*ultrastructure Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
24/01/2008 15:24
Dernière modification de la notice
03/03/2018 13:34
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