Ultrastructure of retinal ganglion cell death after axotomy in chick embryos.

Détails

ID Serval
serval:BIB_0A73366041EA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ultrastructure of retinal ganglion cell death after axotomy in chick embryos.
Périodique
Journal of Comparative Neurology
Auteur(s)
Borsello T., Mottier V., Castagné V., Clarke P.G.H.
ISSN
0021-9967
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
453
Numéro
4
Pages
361-371
Langue
anglais
Notes
SAPHIRID:48837
Résumé
Axotomy often leads to neuronal death, which occurs after a particularly short delay in immature animals. Tectal lesions were made in embryonic day (E) 12 chick embryos, thereby axotomizing the retinal ganglion cells of the contralateral eye, which then died within 3 days. We here describe the ultrastructural changes in the axotomized ganglion cells. The main changes were nuclear invagination and type 3B (cytoplasmic type) cell death characterized by dilation of the perinuclear space, endoplasmic reticulum, and Golgi apparatus. However, nuclear invagination was never seen in type 3B dying cells. All the axotomy-induced retinal ganglion cell death appears to have been of type 3B; apoptosis was not induced by axotomy, as was confirmed by additional light microscopic experiments showing that it did not increase the frequency of apoptotic markers revealed by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (the TUNEL method) labeling and immunoreactivity for activated caspase-3. However, the latter methods did show small numbers of apoptotic cells dying naturally even in control retinas. After the death of the axotomized ganglion cells, they were phagocytosed mainly in Müller processes. The present findings open up the chick tectal lesion model as a system for analyzing type 3B neuronal death in vivo.
Mots-clé
Animals, Axotomy, Cell Death/physiology, Chick Embryo, Retinal Ganglion Cells/ultrastructure
Pubmed
Web of science
Création de la notice
05/02/2008 14:38
Dernière modification de la notice
03/03/2018 13:34
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