Article: article from journal or magazin.
Choice of anticoagulant can influence the analysis using fluorescence in situ hybridization of fetal cells enriched from maternal blood
Journal Article --- Old month value: Mar
A notable degree of research attention is being focused on the use of fetal cells enriched from the blood of pregnant women as a non-invasive means of prenatal diagnosis. By using magnetic activated cells sorting (MACS) and fluorescence in situ hybridization (FISH), we have examined the efficacy of enriched fetal cells in determining fetal sex. An unexpected finding of this investigation was that the sensitivity of this analysis was influenced by the anticoagulant used to treat the maternal blood samples. As such, samples treated with heparin showed significantly lower detection rates than samples chelated with EDTA.
Anticoagulants/*pharmacology Blood Cells/cytology/*drug effects Blood Specimen Collection/methods Cell Death Edetic Acid/pharmacology Female Fetus/*cytology Heparin/pharmacology Humans Immunomagnetic Separation *In Situ Hybridization, Fluorescence Male Pregnancy Sensitivity and Specificity
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