Choice of anticoagulant can influence the analysis using fluorescence in situ hybridization of fetal cells enriched from maternal blood

Détails

ID Serval
serval:BIB_09ED7B07499D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Choice of anticoagulant can influence the analysis using fluorescence in situ hybridization of fetal cells enriched from maternal blood
Périodique
Prenatal Diagnosis
Auteur(s)
Brombacher  V., Kiefer  V., Troeger  C., Vial  Y., Minderer  S., Holzgreve  W., Hahn  S.
ISSN
0197-3851 (Print)
Statut éditorial
Publié
Date de publication
03/2000
Volume
20
Numéro
3
Pages
257-9
Notes
Journal Article --- Old month value: Mar
Résumé
A notable degree of research attention is being focused on the use of fetal cells enriched from the blood of pregnant women as a non-invasive means of prenatal diagnosis. By using magnetic activated cells sorting (MACS) and fluorescence in situ hybridization (FISH), we have examined the efficacy of enriched fetal cells in determining fetal sex. An unexpected finding of this investigation was that the sensitivity of this analysis was influenced by the anticoagulant used to treat the maternal blood samples. As such, samples treated with heparin showed significantly lower detection rates than samples chelated with EDTA.
Mots-clé
Anticoagulants/*pharmacology Blood Cells/cytology/*drug effects Blood Specimen Collection/methods Cell Death Edetic Acid/pharmacology Female Fetus/*cytology Heparin/pharmacology Humans Immunomagnetic Separation *In Situ Hybridization, Fluorescence Male Pregnancy Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 12:17
Dernière modification de la notice
03/03/2018 13:33
Données d'usage