Article: article from journal or magazin.
Expression of the tumor necrosis factor locus is not necessary for the cytolytic activity of T lymphocytes
Journal of Immunology
Journal Article --- Old month value: Mar 15
In order to test whether tumor necrosis factors alpha (TNF-alpha) or beta (TNF-beta, also known as lymphotoxin) are involved in the lysis of target cells by cytolytic T lymphocytes, we probed for the presence of the TNF mRNAs in several quiescent and activated CTL clones. No TNF mRNA could be found in constitutively cytolytic Lyt-2+ clones, and only two out of three clones tested accumulated TNF mRNA after stimulation with phorbol myristate acetate and ionomycin. Of two L3T4+ clones that can be induced to become cytolytic by a combination of antigen and IL-1, only one accumulated TNF-beta mRNA in the process. The PC60 rat X mouse T cell hybrid, which becomes cytolytic in response to a combination of IL-1 and IL-2, also failed to accumulate TNF mRNA after stimulation with these agents. Our results strongly suggest that TNF-alpha or -beta are not necessary agents of the cytolytic activity exhibited by antigen-specific T lymphocytes.
Animals Cell Line *Cytotoxicity, Immunologic Ethers/pharmacology Hybridomas/drug effects/metabolism Interleukin-1/pharmacology Ionomycin Mice RNA, Messenger/analysis Rats T-Lymphocytes, Cytotoxic/drug effects/*immunology Tetradecanoylphorbol Acetate/pharmacology Tumor Necrosis Factor-alpha/biosynthesis/genetics/*physiology
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