Expression of Cre recombinase in pigment cells

Détails

ID Serval
serval:BIB_0927AF1EC235
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of Cre recombinase in pigment cells
Périodique
Pigment Cell Research
Auteur(s)
Guyonneau  L., Rossier  A., Richard  C., Hummler  E., Beermann  F.
ISSN
0893-5785 (Print)
Statut éditorial
Publié
Date de publication
08/2002
Volume
15
Numéro
4
Pages
305-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
Conditional gene targeting using the Cre/loxP system enables specific deletion of a gene in a tissue of interest. For application of Cre-mediated recombination in pigment cells, Cre expression has to be targeted to pigment cells in transgenic mice. So far, no pigment cell-specific Cre transgenic line has been reported and we present and discuss our first results on use of Cre recombinase in pigment cells. A construct was generated where Cre recombinase is controlled by the promoter of the mouse dopachrome tautomerase (Dct) gene. The construct was functionally tested in vitro and introduced into mice. Following breeding to two reporter mouse strains, we detected Cre recombinase activity in telencephalon, melanoblasts, and retinal pigment epithelium (RPE). Our data demonstrate the feasibility of pigment cell-specific Cre/loxP-mediated recombination.
Mots-clé
Animals Cells, Cultured Codon, Terminator/genetics Female Gene Targeting/*methods Green Fluorescent Proteins Integrases/*diagnostic use/*genetics Intramolecular Oxidoreductases/genetics Luminescent Proteins/genetics Male Melanins/biosynthesis/*genetics Melanocytes/cytology/*enzymology Mice Mice, Transgenic Pigment Epithelium of Eye/cytology/*enzymology Promoter Regions (Genetics)/genetics Recombinant Fusion Proteins/genetics Viral Proteins/*diagnostic use/*genetics
Pubmed
Web of science
Création de la notice
24/01/2008 13:42
Dernière modification de la notice
03/03/2018 13:31
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