Mutation screening of the glutamate cysteine ligase modifier (GCLM) gene in patients with schizophrenia.

Details

Serval ID
serval:BIB_08D720EF4F22
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mutation screening of the glutamate cysteine ligase modifier (GCLM) gene in patients with schizophrenia.
Journal
Psychiatric genetics
Author(s)
Butticaz C., Werge T., Beckmann J.S., Cuénod M., Do K.Q., Rivolta C.
ISSN
1473-5873[electronic]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
19
Number
4
Pages
201-8
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
BACKGROUND: Experimental evidences show that glutathione and its rate-limiting synthesizing enzyme, the glutamate-cysteine ligase (GCL), are involved in the pathogenesis of schizophrenia. Furthermore, genetic association has been previously reported between two single nucleotide polymorphisms lying in noncoding regions of glutamate cysteine ligase modifier (GCLM) gene, which specifies for the modifier subunit of GCL and schizophrenia. OBJECTIVE: We wanted to investigate the presence of GCLM true functional mutations, likely in linkage disequilibrium with the previously identified single nucleotide polymorphism alleles, in the same set of cases that allowed the detection of the original association signal. METHODS: We screened all the coding regions of GCLM and their intronic flanking vicinities in 353 patients with schizophrenia by direct DNA sequencing. RESULTS: Ten sequence variations were identified, five of which were not previously described. None of these DNA changes was within the GCLM coding sequence and in-silico analysis failed to indicate functional impairment induced by these variations. Furthermore, screening of normal controls and downstream statistical analyses revealed no significant relationship of any of these DNA variants with schizophrenia. CONCLUSION: It is unlikely that functional mutations in the GCLM gene could play a major role in genetic predisposition to schizophrenia and further studies will be required to assess its etiological function in the disease.
Pubmed
Web of science
Create date
13/07/2009 9:54
Last modification date
20/08/2019 12:31
Usage data