Differential peptide binding to CD40 evokes counteractive responses.

Détails

ID Serval
serval:BIB_08C2F641969D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differential peptide binding to CD40 evokes counteractive responses.
Périodique
Human Immunology
Auteur(s)
Khan S., Alonso L., Roduit C., Bandyopadhyay S., Singh S., Saha S., Tacchini-Cottier F., Roy S., Dietler G., Kasas S., Das P., Krishnasastry M.V., Saha B.
ISSN
1879-1166 (Electronic)
ISSN-L
0198-8859
Statut éditorial
Publié
Date de publication
2012
Volume
73
Numéro
5
Pages
465-469
Langue
anglais
Résumé
The antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD40 function remains unknown. Here, we investigated whether such duality depends on ligand binding. Based on CD40 binding, we identifed two dodecameric peptides, peptide-7 and peptide-19, from the phage peptide library. Peptide-7 induces IL-10 and increases Leishmania donovani infection in macrophages, whereas peptide-19 induces IL-12 and reduces L. donovani infection. CD40-peptide interaction analyses by surface plasmon resonance and atomic force microscopy suggest that the functional differences are not associated with the studied interaction parameters. The molecular dynamic simulation of the CD40-peptides interaction suggests that these two peptides bind to two different places on CD40. Thus, we suggest for the first time that differential binding of the ligands imparts functional duality to CD40.
Mots-clé
Amino Acid Sequence, Antigens, CD40/immunology, Antigens, CD40/metabolism, Binding Sites, Cells, Cultured, Humans, Interleukin-10/immunology, Interleukin-10/metabolism, Interleukin-12/immunology, Interleukin-12/metabolism, Leishmania donovani/immunology, Leishmaniasis, Visceral/immunology, Leishmaniasis, Visceral/parasitology, Macrophages/drug effects, Macrophages/immunology, Microscopy, Atomic Force, Molecular Dynamics Simulation, Molecular Sequence Data, Oligopeptides/immunology, Oligopeptides/metabolism, Peptide Library, Protein Binding, Surface Plasmon Resonance
Pubmed
Web of science
Création de la notice
09/06/2012 19:08
Dernière modification de la notice
20/08/2019 13:31
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