Early markers for myocardial ischemia and sudden cardiac death.

Détails

ID Serval
serval:BIB_0898510C2624
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Early markers for myocardial ischemia and sudden cardiac death.
Périodique
International Journal of Legal Medicine
Auteur(s)
Sabatasso S., Mangin P., Fracasso T., Moretti M., Docquier M., Djonov V.
ISSN
1437-1596 (Electronic)
ISSN-L
0937-9827
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
130
Numéro
5
Pages
1265-1280
Langue
anglais
Notes
Publication types: ARTICLEPublication Status: ppublish
Résumé
The post-mortem diagnosis of acute myocardial ischemia remains a challenge for both clinical and forensic pathologists. We performed an experimental study (ligation of left anterior descending coronary artery in rats) in order to identify early markers of myocardial ischemia, to further apply to forensic and clinical pathology in cases of sudden cardiac death. Using immunohistochemistry, Western blots, and gene expression analyses, we investigated a number of markers, selected among those which are currently used in emergency departments to diagnose myocardial infarction and those which are under investigation in basic research and autopsy pathology studies on cardiovascular diseases. The study was performed on 44 adult male Lewis rats, assigned to three experimental groups: control, sham-operated, and operated. The durations of ischemia ranged between 5 min and 24 h. The investigated markers were troponins I and T, myoglobin, fibronectin, C5b-9, connexin 43 (dephosphorylated), JunB, cytochrome c, and TUNEL staining. The earliest expressions (≤30 min) were observed for connexin 43, JunB, and cytochrome c, followed by fibronectin (≤1 h), myoglobin (≤1 h), troponins I and T (≤1 h), TUNEL (≤1 h), and C5b-9 (≤2 h). By this investigation, we identified a panel of true early markers of myocardial ischemia and delineated their temporal evolution in expression by employing new technologies for gene expression analysis, in addition to traditional and routine methods (such as histology and immunohistochemistry). Moreover, for the first time in the autopsy pathology field, we identified, by immunohistochemistry, two very early markers of myocardial ischemia: dephosphorylated connexin 43 and JunB.
Pubmed
Web of science
Création de la notice
11/07/2016 11:01
Dernière modification de la notice
03/03/2018 13:30
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