The Role of PPARβ/δ in Melanoma Metastasis.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_08607DDF85B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The Role of PPARβ/δ in Melanoma Metastasis.
Périodique
International journal of molecular sciences
Auteur(s)
Lim JCW, Kwan Y.P., Tan M.S., Teo MHY, Chiba S., Wahli W., Wang X.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Statut éditorial
Publié
Date de publication
20/09/2018
Peer-reviewed
Oui
Volume
19
Numéro
10
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Peroxisome proliferator⁻activated receptor (PPAR) β/δ, a ligand-activated transcription factor, is involved in diverse biological processes including cell proliferation, cell differentiation, inflammation and energy homeostasis. Besides its well-established roles in metabolic disorders, PPARβ/δ has been linked to carcinogenesis and was reported to inhibit melanoma cell proliferation, anchorage-dependent clonogenicity and ectopic xenograft tumorigenicity. However, PPARβ/δ's role in tumour progression and metastasis remains controversial.
In the present studies, the consequence of PPARβ/δ inhibition either by global genetic deletion or by a specific PPARβ/δ antagonist, 10h, on malignant transformation of melanoma cells and melanoma metastasis was examined using both in vitro and in vivo models.
Our study showed that 10h promotes epithelial-mesenchymal transition (EMT), migration, adhesion, invasion and trans-endothelial migration of mouse melanoma B16/F10 cells. We further demonstrated an increased tumour cell extravasation in the lungs of wild-type mice subjected to 10h treatment and in Pparβ/δ <sup>-/-</sup> mice in an experimental mouse model of blood-borne pulmonary metastasis by tail vein injection. This observation was further supported by an increased tumour burden in the lungs of Pparβ/δ <sup>-/-</sup> mice as demonstrated in the same animal model.
These results indicated a protective role of PPARβ/δ in melanoma progression and metastasis.
Mots-clé
Animals, Cell Line, Tumor, Epithelial-Mesenchymal Transition/genetics, Melanoma/genetics, Melanoma/pathology, Mice, Neoplasm Invasiveness/genetics, Neoplasm Metastasis/genetics, Neoplasm Metastasis/pathology, PPAR delta/genetics, PPAR delta/metabolism, PPAR delta/physiology, PPAR-beta/genetics, PPAR-beta/metabolism, PPAR-beta/physiology, EMT, invasion, melanoma, metastasis, migration, peroxisome proliferator–activated receptor β/δ
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/10/2018 11:00
Dernière modification de la notice
21/08/2019 7:08
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