Ly 2 positive cytotoxic T lymphocytes, whether specific or non-specific in lytic activity, may express a large, granular, vacuolated lymphocyte morphology.

Détails

ID Serval
serval:BIB_084687BA5C8D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ly 2 positive cytotoxic T lymphocytes, whether specific or non-specific in lytic activity, may express a large, granular, vacuolated lymphocyte morphology.
Périodique
Australian Journal of Experimental Biology and Medical Science
Auteur(s)
Wilson A., Scollay R., Abbot A.P., Shortman K.
ISSN
0004-945X (Print)
ISSN-L
0004-945X
Statut éditorial
Publié
Date de publication
1984
Volume
62 ( Pt 4)
Pages
381-401
Langue
anglais
Résumé
Cytotoxic clones consisting entirely of large, vacuolated granular lymphocytes (LGL) are produced with high frequency when individual purified mature Ly 2+ T cells are cultured at limit dilution in the presence of concanavalin A and irradiated spleen filler cells. Similar cells are produced in cultures of Ly 2- T cells but in lower proportion, the level of granular lymphocytes amongst the largely non-cytotoxic, Ly 2- L3T4+ product cells being only 20%. A proportion (15%) of LGL are also found in conventional mixed lymphocyte cultures. In the limit-dilution cultures of Ly 2+ T cells the LGL originate from the single precursor cell and not from the irradiated filler cells. They bear the markers expected of active cytotoxic T lymphocytes (Thy 1++; PNA++; Ly 1+; Ly 2++; L3T4-). Their DNA and chromosome complement appear normal. The granules within the cells are heterogeneous in form, and some resemble in ultrastructure those described in certain cytotoxic, suppressor and NK-cell lines. The granules do not show mast cell staining characteristics. The LGL are not phagocytic. Although LGL are associated with nonspecific cytotoxicity late in culture, the first granular cells appear early (day 3) and dominate in the clones at times when cytolysis is specific. LGL appear to be the normal morphological form of cytotoxic T lymphocytes when grown under these conditions.
Mots-clé
Animals, Antigens, Ly, Clone Cells/immunology, Clone Cells/ultrastructure, Cytoplasmic Granules/ultrastructure, DNA/analysis, Lymphocyte Culture Test, Mixed, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, T-Lymphocytes, Cytotoxic/analysis, T-Lymphocytes, Cytotoxic/immunology, Time Factors, Vacuoles/ultrastructure
Pubmed
Web of science
Création de la notice
10/04/2013 10:29
Dernière modification de la notice
20/08/2019 12:30
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