Transplantation tolerance induced by regulatory T cells: in vivo mechanisms and sites of action.

Détails

ID Serval
serval:BIB_0736F0515625
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transplantation tolerance induced by regulatory T cells: in vivo mechanisms and sites of action.
Périodique
International Immunopharmacology
Auteur(s)
Golshayan D., Wyss J.C., Abulker C.W., Schaefer S.C., Lechler R.I., Lehr H.A., Pascual M.
ISSN
1878-1705
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
9
Numéro
6
Pages
683-688
Langue
anglais
Résumé
The mechanisms by which CD4(+)CD25(+)Foxp3(+) T (Treg) cells regulate effector T cells in a transplantation setting and their in vivo homeostasis still remain to be clarified. Using a mouse adoptive transfer model, we analyzed the in vivo expansion, trafficking, and effector function of alloreactive T cells and donor-specific Treg cells, in response to a full-thickness skin allograft. Fluorescent-labeled CD4(+)CD25(-) and antigen-specific Treg cells were transferred alone or co-injected into syngeneic BALB/c-Nude recipients transplanted with skins from (C57BL/6 x BALB/c) F1 donors. Treg cells divided in vivo, migrated and accumulated in the allograft draining lymph nodes as well as within the graft. The co-transfer of Treg cells did not modify the early activation and homing of CD4(+)CD25(-) T cells in secondary lymphoid organs. However, in the presence of Treg cells, alloreactive CD4(+)CD25(-) T cells produced significantly less IFN-gamma and were present in reduced numbers in the secondary lymphoid organs. Furthermore, time-course studies showed that Treg cells were recruited into the allograft at a very early stage after transplantation and effectively prevented the infiltration of effector T cells. In conclusion, suppression of rejection requires the early recruitment to the site of antigenic challenge of donor-specific Treg cells, which then mainly regulate the effector arm of T cell alloresponses.
Pubmed
Web of science
Création de la notice
20/05/2009 19:15
Dernière modification de la notice
20/08/2019 12:29
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