New route to bacterial production of indigo.

Détails

ID Serval
serval:BIB_06843D3B1A03
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
New route to bacterial production of indigo.
Périodique
Nature Biotechnology
Auteur(s)
Mermod N., Harayama S., Timmis K.N.
ISSN
1087-0156
Statut éditorial
Publié
Date de publication
1986
Peer-reviewed
Oui
Volume
4
Pages
321-324
Langue
anglais
Résumé
Cells of Escherichia coli K-12 containing a cloned fragment of Pseudomonas putida TOL plasmid pWW0 produce the dye indigo. Analysis of the cloned fragment and Tn1000 transposon insertion mutagenesis has identified the xylA gene as being responsible for this phenotype. The xylA gene specifies xylene oxidase, a relaxed specificity enzyme that hydroxylates or monooxygenates toluene and xylenes and their corresponding alcohols. Indole, which is formed from tryptophan by tryptophanase in E. coli, was shown to be a precursor in the reaction sequence leading to indigo formation. These results suggest a novel route for bacterial production of indigo via hydroxylation of indole.
Web of science
Création de la notice
20/03/2008 13:00
Dernière modification de la notice
03/03/2018 13:26
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