Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_0678888C73AE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.
Périodique
Nucleic Acids Research
Auteur(s)
Benedetti F., Racko D., Dorier J., Burnier Y., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
45
Numéro
17
Pages
9850-9859
Langue
anglais
Résumé
The question of how self-interacting chromatin domains in interphase chromosomes are structured and generated dominates current discussions on eukaryotic chromosomes. Numerical simulations using standard polymer models have been helpful in testing the validity of various models of chromosome organization. Experimental contact maps can be compared with simulated contact maps and thus verify how good is the model. With increasing resolution of experimental contact maps, it became apparent though that active processes need to be introduced into models to recapitulate the experimental data. Since transcribing RNA polymerases are very strong molecular motors that induce axial rotation of transcribed DNA, we present here models that include such rotational motors. We also include into our models swivels and sites for intersegmental passages that account for action of DNA topoisomerases releasing torsional stress. Using these elements in our models, we show that transcription-induced supercoiling generated in the regions with divergent-transcription and supercoiling relaxation occurring between these regions are sufficient to explain formation of self-interacting chromatin domains in chromosomes of fission yeast (S. pombe).

Mots-clé
Biomechanical Phenomena, Chromatin/chemistry, Chromatin/metabolism, Chromosomes, Fungal/chemistry, Chromosomes, Fungal/metabolism, DNA Topoisomerases/chemistry, DNA Topoisomerases/genetics, DNA Topoisomerases/metabolism, DNA, Fungal/chemistry, DNA, Fungal/genetics, DNA, Fungal/metabolism, DNA, Superhelical/chemistry, DNA, Superhelical/genetics, DNA, Superhelical/metabolism, DNA-Directed RNA Polymerases/chemistry, DNA-Directed RNA Polymerases/genetics, DNA-Directed RNA Polymerases/metabolism, Gene Expression Regulation, Fungal, Molecular Dynamics Simulation, Rotation, Schizosaccharomyces/genetics, Schizosaccharomyces/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/10/2017 12:45
Dernière modification de la notice
20/08/2019 12:28
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