2C-Cas9: a versatile tool for clonal analysis of gene function.

Détails

ID Serval
serval:BIB_066EF01D78EF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
2C-Cas9: a versatile tool for clonal analysis of gene function.
Périodique
Genome Research
Auteur(s)
Di Donato V., De Santis F., Auer T.O., Testa N., Sánchez-Iranzo H., Mercader N., Concordet J.P., Del Bene F.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
2016
Volume
26
Numéro
5
Pages
681-692
Langue
anglais
Résumé
CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of gene inactivation. Here, we show that tissue-specific gene disruption can be achieved by driving Cas9 expression with the Gal4/UAS system. Furthermore, by combining the Gal4/UAS and Cre/loxP systems, we establish a versatile tool to genetically label mutant cell clones, enabling their phenotypic analysis. Our technique has the potential to be applied to diverse model organisms, enabling tissue-specific loss-of-function and phenotypic characterization of live and fixed tissues.
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/05/2016 8:58
Dernière modification de la notice
20/08/2019 12:28
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