Transfer of ultrasmall iron oxide nanoparticles from human brain-derived endothelial cells to human glioblastoma cells.

Details

Serval ID
serval:BIB_065C1E05DC80
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transfer of ultrasmall iron oxide nanoparticles from human brain-derived endothelial cells to human glioblastoma cells.
Journal
ACS Applied Materials and Interfaces
Author(s)
Halamoda Kenzaoui B., Angeloni S., Overstolz T., Niedermann P., Chapuis Bernasconi C., Liley M., Juillerat-Jeanneret L.
ISSN
1944-8252 (Electronic)
ISSN-L
1944-8244
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
5
Number
9
Pages
3581-3586
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Nanoparticles (NPs) are being used or explored for the development of biomedical applications in diagnosis and therapy, including imaging and drug delivery. Therefore, reliable tools are needed to study the behavior of NPs in biological environment, in particular the transport of NPs across biological barriers, including the blood-brain tumor barrier (BBTB), a challenging question. Previous studies have addressed the translocation of NPs of various compositions across cell layers, mostly using only one type of cells. Using a coculture model of the human BBTB, consisting in human cerebral endothelial cells preloaded with ultrasmall superparamagnetic iron oxide nanoparticles (USPIO NPs) and unloaded human glioblastoma cells grown on each side of newly developed ultrathin permeable silicon nitride supports as a model of the human BBTB, we demonstrate for the first time the transfer of USPIO NPs from human brain-derived endothelial cells to glioblastoma cells. The reduced thickness of the permeable mechanical support compares better than commercially available polymeric supports to the thickness of the basement membrane of the cerebral vascular system. These results are the first report supporting the possibility that USPIO NPs could be directly transferred from endothelial cells to glioblastoma cells across a BBTB. Thus, the use of such ultrathin porous supports provides a new in vitro approach to study the delivery of nanotherapeutics to brain cancers. Our results also suggest a novel possibility for nanoparticles to deliver therapeutics to the brain using endothelial to neural cells transfer.
Keywords
Nanoparticles, Cell-cell transfer, Porous ultrathin silicon nitride membrane, Human blood-brain tumor barrier, Therapeutics delivery
Pubmed
Web of science
Create date
14/05/2013 10:28
Last modification date
20/08/2019 12:28
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