Article: article from journal or magazin.
T-type Ca2+ channels, SK2 channels and SERCAs gate sleep-related oscillations in thalamic dendrites.
T-type Ca2+ channels (T channels) underlie rhythmic burst discharges during neuronal oscillations that are typical during sleep. However, the Ca2+-dependent effectors that are selectively regulated by T currents remain unknown. We found that, in dendrites of nucleus reticularis thalami (nRt), intracellular Ca2+ concentration increases were dominated by Ca2+ influx through T channels and shaped rhythmic bursting via competition between Ca2+-dependent small-conductance (SK)-type K+ channels and Ca2+ uptake pumps. Oscillatory bursting was initiated via selective activation of dendritically located SK2 channels, whereas Ca2+ sequestration by sarco/endoplasmic reticulum Ca2+-ATPases (SERCAs) and cumulative T channel inactivation dampened oscillations. Sk2-/- (also known as Kcnn2) mice lacked cellular oscillations, showed a greater than threefold reduction in low-frequency rhythms in the electroencephalogram of non-rapid-eye-movement sleep and had disrupted sleep. Thus, the interplay of T channels, SK2 channels and SERCAs in nRt dendrites comprises a specialized Ca2+ signaling triad to regulate oscillatory dynamics related to sleep.
Anesthetics, Local/pharmacology, Animals, Animals, Newborn, Apamin/pharmacology, Biological Clocks/physiology, Calcium/metabolism, Calcium Channel Blockers/pharmacology, Dendrites/drug effects, Dendrites/metabolism, Electric Stimulation/methods, Electroencephalography/methods, Enzyme Inhibitors/pharmacology, Female, Indoles/pharmacology, Membrane Potentials/drug effects, Membrane Potentials/physiology, Mibefradil/pharmacology, Mice, Mice, Knockout, Midline Thalamic Nuclei/cytology, Neurons/cytology, Neurons/drug effects, Patch-Clamp Techniques, Sarcoplasmic Reticulum Calcium-Transporting ATPases/physiology, Sleep/physiology, Small-Conductance Calcium-Activated Potassium Channels/deficiency, Small-Conductance Calcium-Activated Potassium Channels/physiology, Tetrodotoxin/pharmacology, Walking/physiology
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