Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing

Détails

ID Serval
serval:BIB_061057DE35B9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing
Périodique
PCR Methods and Applications
Auteur(s)
Bautista  D., Chaubert  P., Bertoncini  J., Benhattar  J.
ISSN
1054-9803 (Print)
Statut éditorial
Publié
Date de publication
1994
Volume
4
Numéro
2
Pages
76-79
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
A simple procedure for direct sequencing of double-stranded PCR products by the dideoxy-termination method has been developed using biotinylated sequencing primers. Sequences of the p53 gene have been obtained from DNA extracted from frozen and formalin-fixed paraffin-embedded cancer tissues. Detection of sequencing ladders was done with chemiluminescent or colorimetric techniques. Both are highly sensitive, but colorimetric detection is less prone to diffusion artifacts, which are common in G-rich regions. Use of this nonradioactive PCR-sequencing protocol allowed rapid and simple determination of p53 gene alterations in human tumors
Mots-clé
Base Sequence/Chemiluminescent Measurements/Colorimetry/DNA Primers/Electrophoresis,Agar Gel/Genes,p53/Humans/Molecular Sequence Data/Mutation/Neoplasms/genetics/Polymerase Chain Reaction/Sequence Analysis,DNA/methods/Research
Pubmed
Création de la notice
29/01/2008 19:34
Dernière modification de la notice
03/03/2018 13:25
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