Ultra-high-resolution 3D imaging of atherosclerosis in mice with synchrotron differential phase contrast: a proof of concept study.

Détails

Ressource 1Télécharger: BIB_05F33CDA64DE.P001.pdf (1682.71 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_05F33CDA64DE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ultra-high-resolution 3D imaging of atherosclerosis in mice with synchrotron differential phase contrast: a proof of concept study.
Périodique
Scientific Reports
Auteur(s)
Bonanno G., Coppo S., Modregger P., Pellegrin M., Stuber A., Stampanoni M., Mazzolai L., Stuber M., van Heeswijk R.B.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
5
Pages
11980
Langue
anglais
Résumé
The goal of this study was to investigate the performance of 3D synchrotron differential phase contrast (DPC) imaging for the visualization of both macroscopic and microscopic aspects of atherosclerosis in the mouse vasculature ex vivo. The hearts and aortas of 2 atherosclerotic and 2 wild-type control mice were scanned with DPC imaging with an isotropic resolution of 15 μm. The coronary artery vessel walls were segmented in the DPC datasets to assess their thickness, and histological staining was performed at the level of atherosclerotic plaques. The DPC imaging allowed for the visualization of complex structures such as the coronary arteries and their branches, the thin fibrous cap of atherosclerotic plaques as well as the chordae tendineae. The coronary vessel wall thickness ranged from 37.4 ± 5.6 μm in proximal coronary arteries to 13.6 ± 3.3 μm in distal branches. No consistent differences in coronary vessel wall thickness were detected between the wild-type and atherosclerotic hearts in this proof-of-concept study, although the standard deviation in the atherosclerotic mice was higher in most segments, consistent with the observation of occasional focal vessel wall thickening. Overall, DPC imaging of the cardiovascular system of the mice allowed for a simultaneous detailed 3D morphological assessment of both large structures and microscopic details.
Pubmed
Web of science
Création de la notice
01/08/2015 9:45
Dernière modification de la notice
03/03/2018 13:25
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