Proteomic analyses of amniotic fluid: potential applications in health and diseases

Details

Serval ID
serval:BIB_05D2D4554F0D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteomic analyses of amniotic fluid: potential applications in health and diseases
Journal
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Author(s)
Queloz  P. A., Crettaz  D., Thadikkaran  L., Sapin  V., Gallot  D., Jani  J., Deprest  J., Lemery  D., Barelli  S., Tissot  J. D.
ISSN
1570-0232 (Print)
Publication state
Published
Issued date
05/2007
Volume
850
Number
1-2
Pages
336-42
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 1
Abstract
Amniotic fluid (AF) is a potential source of biomarkers for many disorders which may occur during pregnancy. The purpose of this study was to evaluate the place of two-dimensional gel electrophoresis (2-DE) technologies to compare AF in both normal and pathological situations. Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE; Ettan DIGE) as well as two-dimensional gel electrophoresis and silver staining followed by image analysis were used. Differentially expressed proteins were identified by mass spectrometry. This approach was used to study electrophoregrams of normal AF obtained at 17 weeks of gestation and at term, as well as AF from fetuses presenting with congenital diaphragmatic hernia. Finally, the potential of two-dimensional electrophoresis was assessed by studying the protein profile of plasma containing AF proteins in a model of premature rupture of the membranes (PROM). Our results clearly show that two-dimensional electrophoresis technologies still have place for analyzing biological fluids such as AF.
Keywords
Amino Acid Sequence Amniotic Fluid/*chemistry Electrophoresis, Gel, Two-Dimensional Female Hernia, Diaphragmatic/congenital/diagnosis Humans Molecular Sequence Data Pregnancy Pregnancy Trimester, Second Prenatal Diagnosis/methods *Proteome Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 12:27
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