Article: article from journal or magazin.
Quality control limits for fluconazole disk susceptibility tests on Mueller-Hinton agar with glucose and methylene blue
Journal of Clinical Microbiology
Journal Article --- Old month value: Jul
An international collaborative study was performed in order to propose quality control limits for fluconazole disk diffusion susceptibility tests on Mueller-Hinton agar with 2% glucose and 0.5 micro g of methylene blue per ml. The supplements may be added before autoclaving the agar, or Mueller-Hinton agar plates may be flooded with a glucose-methylene blue solution. Replicate tests on both types of agar plates generated data to propose zone size limits for tests of Candida parapsilosis ATCC 22019 (22 to 33 mm), C. tropicalis ATCC 750 (26 to 37 mm), and C. albicans ATCC 90028 (28 to 39 mm). C. krusei ATCC 6258 was not useful for this purpose.
Agar Antifungal Agents/*pharmacology Candida/*drug effects/growth & development Candida albicans/drug effects/growth & development Culture Media Fluconazole/*pharmacology Glucose Humans International Cooperation Laboratories Methylene Blue Microbial Sensitivity Tests/*standards Quality Control
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