Quantification of clenbuterol at trace level in human urine by ultra-high pressure liquid chromatography-tandem mass spectrometry.

Détails

ID Serval
serval:BIB_04EB599A9A44
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantification of clenbuterol at trace level in human urine by ultra-high pressure liquid chromatography-tandem mass spectrometry.
Périodique
Journal of Chromatography. A
Auteur(s)
Nicoli R., Petrou M., Badoud F., Dvorak J., Saugy M., Baume N.
ISSN
1873-3778 (Electronic)
ISSN-L
0021-9673
Statut éditorial
Publié
Date de publication
2013
Volume
1292
Pages
142-150
Langue
anglais
Notes
Publication types: Journal Article ; Validation Studies
Publication Status: ppublish
Résumé
Clenbuterol is a β2 agonist agent with anabolic properties given by the increase in the muscular mass in parallel to the decrease of the body fat. For this reason, the use of clenbuterol is forbidden by the World Anti-Doping Agency (WADA) in the practice of sport. This compound is of particular interest for anti-doping authorities and WADA-accredited laboratories due to the recent reporting of risk of unintentional doping following the eating of meat contaminated with traces of clenbuterol in some countries. In this work, the development and the validation of an ultra-high pressure liquid chromatography coupled to electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method for the quantification of clenbuterol in human urine is described. The analyte was extracted from urine samples by liquid-liquid extraction (LLE) in basic conditions using tert butyl-methyl ether (TBME) and analyzed by UHPLC-MS/MS with a linear gradient of acetonitrile in 9min only. The simple and rapid method presented here was validated in compliance with authority guidelines and showed a limit of quantification at 5pg/mL and a linearity range from 5pg/mL to 300pg/mL. Good trueness (85.8-105%), repeatability (5.7-10.6% RSD) and intermediate precision (5.9-14.9% RSD) results were obtained. The method was then applied to real samples from eighteen volunteers collecting urines after single oral doses administration (1, 5 and 10μg) of clenbuterol-enriched yogurts.
Mots-clé
Adrenergic beta-Agonists/urine, Chromatography, High Pressure Liquid/methods, Clenbuterol/urine, Doping in Sports, Food Contamination/analysis, Humans, Liquid-Liquid Extraction/methods, Male, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/methods, Tandem Mass Spectrometry/methods
Pubmed
Web of science
Création de la notice
12/02/2013 16:55
Dernière modification de la notice
03/03/2018 13:23
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