Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-alpha in humans: no alteration in adipose tissue of obese and NIDDM patients.
Details
Serval ID
serval:BIB_04E268818865
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-alpha in humans: no alteration in adipose tissue of obese and NIDDM patients.
Journal
Diabetes
ISSN
0012-1797 (Print)
ISSN-L
0012-1797
Publication state
Published
Issued date
1997
Volume
46
Number
8
Pages
1319-1327
Language
english
Abstract
Members of the peroxisome proliferator-activated receptor (PPAR) family might be involved in pathologies with altered lipid metabolism. They participate in the control of the expression of genes involved in lipid metabolism and adipocyte differentiation. In addition, thiazolidinediones improve insulin resistance in vivo by activating PPAR gamma. However, little is known regarding their tissue distribution and relative expression in humans. Using a quantitative and sensitive reverse transcription (RT)-competitive polymerase chain reaction (PCR) assay, we determined the distribution and relative mRNA expression of the four PPARs (alpha,beta, gamma1, and gamma2) and liver X receptor-alpha (LXR alpha) in the main tissues implicated in lipid metabolism. PPAR alpha and LXR alpha were mainly expressed in liver, while PPAR gamma1 predominated in adipose tissue and large intestine. We found that PPAR gamma2 mRNA was a minor isoform, even in adipose tissue, thus causing question of its role in humans. PPAR beta mRNA was present in all the tissues tested at low levels. In addition, PPAR gamma mRNA was barely detectable in skeletal muscle, suggesting that improvement of insulin resistance with thiazolidinediones may not result from a direct effect of these agents on PPAR gamma in muscle. Obesity and NIDDM were not associated with change in PPARs and LXR alpha expression in adipose tissue. The mRNA levels of PPAR gamma1, the predominant form in adipocytes, did not correlate with BMI, leptin mRNA levels, or fasting insulinemia in 29 subjects with various degrees of obesity. These results indicated that obesity is not associated with alteration in PPAR gene expression in abdominal subcutaneous adipose tissue in humans.
Keywords
Adipocytes/chemistry, Adipocytes/cytology, Adipose Tissue/chemistry, Adipose Tissue/metabolism, Base Sequence, Biopsy, Cells, Cultured, Cohort Studies, DNA Primers/chemistry, DNA-Binding Proteins, Diabetes Mellitus, Type 2/genetics, Diabetes Mellitus, Type 2/metabolism, Female, Gene Expression/genetics, Humans, Intestine, Large/chemistry, Intestine, Large/pathology, Intestine, Small/chemistry, Intestine, Small/pathology, Kidney/chemistry, Kidney/pathology, Liver/chemistry, Liver/pathology, Male, Muscle, Skeletal/chemistry, Muscle, Skeletal/metabolism, Nuclear Proteins/genetics, Obesity/genetics, Obesity/metabolism, Orphan Nuclear Receptors, Polymerase Chain Reaction, RNA, Messenger/analysis, RNA, Messenger/genetics, Receptors, Cytoplasmic and Nuclear/genetics, Transcription Factors/genetics
Pubmed
Web of science
Create date
07/03/2013 17:12
Last modification date
20/08/2019 13:26
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