Article: article from journal or magazin.
Homologous domains of the largest subunit of eucaryotic RNA polymerase II are conserved in plants.
Molecular and General Genetics
Genomic and cDNA clones homologous to the RpII215 gene of Drosophila were isolated from Arabidopsis thaliana and assigned to a single copy gene encoding a transcript of 6.8 kb. Nucleotide sequence analysis of Arabidopsis genomic and cDNAs revealed a striking homology to yeast, Caenorhabditis, Drosophila and mouse genes encoding the largest subunit of RNA polymerase II. The Arabidopsis gene rpII215 contains 13 introns, 12 of which interrupt the coding sequence of a protein of 205 kDa. The position of the first intron is conserved between plant and animal genes, while an intron located in the 3' untranslated region of the rpII215 gene is unique to Arabidopsis. Common domains present in all known largest subunits of eucaryotic RNA polymerase II were identified in the predicted sequence of the Arabidopsis RpII215 protein. Both the order and the position of N-terminal Zn2+ finger and of DNA and alpha-amanitin binding motifs are conserved in Arabidopsis. The C-terminal region of the Arabidopsis protein contains 15 consensus and 26 variant YSPTSPS repeats (CTDs). Highly conserved structure among the various C-terminal domains suggests that the largest subunit of RNA polymerase II in plants may also interact with transcription factors and with protein kinases that control the cell cycle as in other organisms.
Amino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Brassica/enzymology, Brassica/genetics, Cloning, Molecular, Drosophila/genetics, Genes, Plant, Introns, Molecular Sequence Data, RNA Polymerase II/genetics, RNA Polymerase II/metabolism, Restriction Mapping, Sequence Homology, Nucleic Acid, Transcription, Genetic
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