Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli.

Détails

ID Serval
serval:BIB_04ADCB482A32
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Competition between SsrA tagging and translational termination at weak stop codons in Escherichia coli.
Périodique
Molecular Microbiology
Auteur(s)
Collier J., Binet E., Bouloc P.
ISSN
0950-382X
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
45
Numéro
3
Pages
745-754
Langue
anglais
Résumé
SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremities. One of them, ribokinase, is expressed from a messenger with a poorly efficient stop codon, leading to translational recoding events. A change in the ribokinase coding sequence from a weak to a strong translational stop sequence (UGAc to UAAu) annihilated SsrA tagging. Translational termination by UGA recruits the translational release factor (RF) 2. We observed that SsrA tagging of ribokinase was inversely correlated with RF2 activity, revealing a dynamic competition between translational termination and SsrA tagging.
Mots-clé
Codon, Terminator, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Phosphotransferases (Alcohol Group Acceptor), Plasmids, Protein Biosynthesis, RNA Processing, Post-Transcriptional, RNA, Bacterial, RNA, Messenger, Regulatory Sequences, Nucleic Acid, Ribosomes
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/02/2009 16:47
Dernière modification de la notice
08/05/2019 13:52
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