Demyelination in brain cell aggregate cultures, induced by a monoclonal antibody against the myelin/oligodendrocyte glycoprotein (MOG).

Détails

ID Serval
serval:BIB_04447BD9E9F5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Demyelination in brain cell aggregate cultures, induced by a monoclonal antibody against the myelin/oligodendrocyte glycoprotein (MOG).
Périodique
Schweizer Archiv für Neurologie und Psychiatrie
Auteur(s)
Honegger P., Matthieu J.M., Lassmann H.
ISSN
0258-7661 (Print)
ISSN-L
0258-7661
Statut éditorial
Publié
Date de publication
1989
Volume
140
Numéro
1
Pages
10-13
Langue
anglais
Résumé
Previous work has shown that aggregate cultures prepared from fetal rat telencephalon and grown in a chemically defined medium offer a useful model to study developmental processes such as myelin synthesis. Since compact myelin is formed in these cultures, we investigated the possibility to use this culture system to study demyelinating mechanisms. In particular, we examined the effect of a monoclonal antibody (8-18C5) directed against the myelin/oligodendrocyte glycoprotein (MOG). We found that addition of anti-MOG antibodies and complement to aggregate cultures led to a highly significant decrease in myelin basic protein (MBP) content and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) specific activity. These results indicate that, in our culture system, anti-MOG antibodies have a strong demyelinating effect.
Mots-clé
Animals, Antibodies, Monoclonal/immunology, Cells, Cultured, Fetus, Galactosylceramides/immunology, Myelin Basic Protein/immunology, Myelin Proteins/immunology, Myelin Sheath/immunology, Myelin-Associated Glycoprotein, Neuroglia/immunology, Oligodendroglia/immunology, Rats, Telencephalon/immunology
Pubmed
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 13:26
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