Measurement of ERK 1/2 in CSF from patients with neuropsychiatric disorders and evidence for the presence of the activated form.

Details

Serval ID
serval:BIB_03F04FC77E20
Type
Article: article from journal or magazin.
Collection
Publications
Title
Measurement of ERK 1/2 in CSF from patients with neuropsychiatric disorders and evidence for the presence of the activated form.
Journal
Journal of Alzheimer's Disease : Jad
Author(s)
Klafki H.W., Lewczuk P., Kamrowski-Kruck H., Maler J.M., Müller K., Peters O., Heuser I., Jessen F., Popp J., Frölich L., Wolf S., Prinz B., Luckhaus C., Schröder J., Pantel J., Gertz H.J., Kölsch H., Müller B.W., Esselmann H., Bibl M., Kornhuber J., Wiltfang J.
ISSN
1875-8908 (Electronic)
ISSN-L
1387-2877
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
18
Number
3
Pages
613-622
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
The clinical diagnosis of neurodegenerative disorders can be supported by soluble biomarkers in cerebrospinal fluid (CSF), such as tau protein, phospho-tau, and amyloid-beta peptides. In particular, increased CSF levels of phospho-tau in Alzheimer's disease appear to reflect disease specific pathological processes. We report here evidence for the presence of soluble MAP-kinase ERK1/2 in a small set of human CSF samples from patients with Alzheimer's disease, frontotemporal degeneration, and mild cognitive impairment. The level of total ERK1/2 in CSF as measured by electrochemiluminescent assay was correlated with that of total tau and phospho-tau. A small fraction of ERK1/2 in a pooled CSF sample was found to be in the doubly phosphorylated (activated) state. Our findings suggest that i) MAP kinase ERK1/2 is apparently released under neurodegenerative conditions in parallel with tau and phospho-tau and ii) in the future, it might be possible to find in CSF samples evidence for disease related alterations in brain kinase signaling pathways by use of highly sensitive and activation-state specific anti-kinase antibodies.
Keywords
Aged, Alzheimer Disease/cerebrospinal fluid, Alzheimer Disease/genetics, Antibodies, Monoclonal, Blotting, Western, Extracellular Signal-Regulated MAP Kinases/cerebrospinal fluid, Extracellular Signal-Regulated MAP Kinases/genetics, Female, Frontotemporal Lobar Degeneration/cerebrospinal fluid, Frontotemporal Lobar Degeneration/genetics, Humans, Immunoprecipitation, Male, Mitogen-Activated Protein Kinase 3/cerebrospinal fluid, Mitogen-Activated Protein Kinase 3/genetics, Severity of Illness Index, tau Proteins/cerebrospinal fluid, tau Proteins/genetics
Pubmed
Create date
29/10/2012 10:58
Last modification date
20/08/2019 12:25
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