Human Fetal Progenitor Tenocytes for Regenerative Medicine.

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Ressource 1Télécharger: BIB_0324ED809217.P001.pdf (1148.15 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_0324ED809217
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human Fetal Progenitor Tenocytes for Regenerative Medicine.
Périodique
Cell Transplantation
Auteur(s)
Grognuz A., Scaletta C., Farron A., Raffoul W., Applegate L.A.
ISSN
1555-3892 (Electronic)
ISSN-L
0963-6897
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
25
Numéro
3
Pages
463-479
Langue
anglais
Résumé
Tendon injuries are very frequent and affect a wide and heterogeneous population. Unfortunately, the healing process is long with outcomes that are not often satisfactory due to fibrotic tissue appearance, which leads to scar and adhesion development. Tissue engineering and cell therapies emerge as interesting alternatives to classical treatments. In this study, we evaluated human fetal progenitor tenocytes (hFPTs) as a potential cell source for treatment of tendon afflictions, as fetal cells are known to promote healing in a scarless regenerative process. hFPTs presented a rapid and stable growth up to passage 9, allowing to create a large cell bank for off-the-shelf availability. hFPTs showed a strong tenogenic phenotype with an excellent stability, even when placed in conditions normally inducing cells to differentiate. The karyotype also indicated a good stability up to passage 12, which is far beyond that necessary for clinical application (passage 6). When placed in coculture, hFPTs had the capacity to stimulate human adult tenocytes (hATs), which are responsible for the deposition of a new extracellular matrix during tendon healing. Finally, it was possible to distribute cells in porous or gel scaffolds with an excellent survival, thus permitting a large variety of applications (from simple injections to grafts acting as filling material). All of these results are encouraging in the development of an off-the-shelf cell source capable of stimulating tendon regeneration for the treatment of tendon injuries.
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/01/2016 12:55
Dernière modification de la notice
20/08/2019 12:25
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