Direct effect of ghrelin on leptin production by cultured rat white adipocytes.

Details

Serval ID
serval:BIB_02BE9A9952A1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct effect of ghrelin on leptin production by cultured rat white adipocytes.
Journal
Obesity
Author(s)
Giovambattista A., Piermaría J., Suescun M.O., Calandra R.S., Gaillard R.C., Spinedi E.
ISSN
1930-7381
Publication state
Published
Issued date
01/2006
Peer-reviewed
Oui
Volume
14
Number
1
Pages
19-27
Language
english
Notes
Publication types: Journal Article
Abstract
OBJECTIVE: Because ghrelin is known to stimulate adipogenesis, we tested whether ghrelin could contribute to the maintenance of homeostasis, directly affecting rat white adipocyte leptin production. RESEARCH METHODS AND PROCEDURES: Isolated retroperitoneal adipocytes were cultured for 0.5 to 48 hours without (baseline) or with (0.001 to 1 nM) ghrelin alone or in combination with insulin (0.01 to 10 nM) or dexamethasone (1 to 100 nM). Adipocytes were also incubated with ghrelin and inhibitors either of RNA (actinomycin D) or protein synthesis (cycloheximide) or with several concentrations (10 to 1000 nM) of a specific ghrelin antagonist. When cultures were terminated, we evaluated adipocyte leptin secretion and ob mRNA expression. RESULTS: Our data indicate that ghrelin directly enhanced adipocyte leptin release and ob mRNA expression, that the leptin-releasing activity of ghrelin was additive to the action of both insulin and dexamethasone and was abrogated by protein synthesis inhibitors, and that effects of ghrelin on adipocyte ob mRNA expression and release were blocked by coincubation with the specific growth hormone secretagogue receptor 1a antagonist. DISCUSSION: Our study supports the ability of ghrelin to enhance white adipose tissue leptin production by a direct receptor-mediated effect. This activity of ghrelin could play a potentially significant role in rapid restoration of homeostasis after food intake.
Keywords
Adipocytes/metabolism, Animals, Cells, Cultured, Cycloheximide/pharmacology, Dactinomycin/pharmacology, Dexamethasone/pharmacology, Dose-Response Relationship, Drug, Drug Synergism, Ghrelin, Insulin/pharmacology, Leptin/biosynthesis, Male, Peptide Hormones/antagonists & inhibitors, Peptide Hormones/pharmacology, Protein Synthesis Inhibitors/pharmacology, RNA, Messenger/metabolism, Rats, Rats, Inbred F344
Pubmed
Web of science
Open Access
Yes
Create date
15/02/2008 16:58
Last modification date
20/08/2019 12:24
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