Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. II: Analysis of biological samples.

Détails

ID Serval
serval:BIB_0249A43DB6AF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. II: Analysis of biological samples.
Périodique
Analytica Chimica Acta
Auteur(s)
Nováková L., Rentsch M., Grand-Guillaume Perrenoud A., Nicoli R., Saugy M., Veuthey J.L., Guillarme D.
ISSN
1873-4324 (Electronic)
ISSN-L
0003-2670
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
853
Pages
647-659
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The potential and applicability of UHPSFC-MS/MS for anti-doping screening in urine samples were tested for the first time. For this purpose, a group of 110 doping agents with diverse physicochemical properties was analyzed using two separation techniques, namely UHPLC-MS/MS and UHPSFC-MS/MS in both ESI+ and ESI- modes. The two approaches were compared in terms of selectivity, sensitivity, linearity and matrix effects. As expected, very diverse retentions and selectivities were obtained in UHPLC and UHPSFC, proving a good complementarity of these analytical strategies. In both conditions, acceptable peak shapes and MS detection capabilities were obtained within 7min analysis time, enabling the application of these two methods for screening purposes. Method sensitivity was found comparable for 46% of tested compounds, while higher sensitivity was observed for 21% of tested compounds in UHPLC-MS/MS and for 32% in UHPSFC-MS/MS. The latter demonstrated a lower susceptibility to matrix effects, which were mostly observed as signal suppression. In the case of UHPLC-MS/MS, more serious matrix effects were observed, leading typically to signal enhancement and the matrix effect was also concentration dependent, i.e., more significant matrix effects occurred at the lowest concentrations.
Pubmed
Web of science
Création de la notice
04/12/2014 15:57
Dernière modification de la notice
03/03/2018 13:17
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