Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.

Détails

Ressource 1Télécharger: elife-45983-v2.pdf (3527.36 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_01CBB053B8AE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.
Périodique
eLife
Auteur(s)
Gerganova V., Floderer C., Archetti A., Michon L., Carlini L., Reichler T., Manley S., Martin S.G.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Statut éditorial
Publié
Date de publication
03/05/2019
Peer-reviewed
Oui
Volume
8
Pages
e45983
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rely on membrane association regulated by a phosphorylation-dephosphorylation cycle and lateral diffusion modulated by clustering. Using quantitative PALM imaging, we find individual Pom1 molecules bind the membrane too transiently to diffuse from pole to mid-cell. Instead, we propose they exchange within longer lived clusters forming the functional gradient unit. An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and buffers the gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool. TIRF imaging of this cortical pool demonstrates more Pom1 overlaps with Cdr2 in short than long cells, consistent with Pom1 inhibition of Cdr2 decreasing with cell growth. Thus, the gradients modulate Pom1 mid-cell levels according to cell size.
Mots-clé
General Biochemistry, Genetics and Molecular Biology, General Immunology and Microbiology, General Neuroscience, General Medicine, S. pombe, cell biology, Pom1 DYRK kinase, cell size, concentration gradient, diffusion, single particle tracking PALM
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/05/2019 16:01
Dernière modification de la notice
20/08/2019 12:24
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