Fungicidal activity of copper-sputtered flexible surfaces under dark and actinic light against azole-resistant Candida albicans and Candida glabrata.

Détails

ID Serval
serval:BIB_0187FC433B8C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fungicidal activity of copper-sputtered flexible surfaces under dark and actinic light against azole-resistant Candida albicans and Candida glabrata.
Périodique
Journal of Photochemistry and Photobiology. B, Biology
Auteur(s)
Ballo MKS, Rtimi S., Kiwi J., Pulgarin C., Entenza J.M., Bizzini A.
ISSN
1873-2682 (Electronic)
ISSN-L
1011-1344
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
174
Pages
229-234
Langue
anglais
Résumé
Candida spp. are able to survive on hospital surfaces and causes healthcare-associated infections (HCAIs). Since surface cleaning and disinfecting interventions are not totally effective to eliminate Candida spp., new approaches should be devised. Copper (Cu) has widely recognized antifungal activity and the use of Cu-sputtered surfaces has recently been proposed to curb the spread of HCAIs. Moreover, the activity of Cu under the action of actinic light remains underexplored. We investigated the antifungal activity of Cu-sputtered polyester surfaces (Cu-PES) against azole-resistant Candida albicans and Candida glabrata under dark and low intensity visible light irradiation (4.65mW/cm(2)). The surface properties of Cu-PES photocatalysts were characterized by diffuse reflectance spectroscopy (DRS) and X-ray fluorescence (XRF). Under dark, Cu-PES showed a fungicidal activity (≥3log10CFU reduction of the initial inoculum) against both C. albicans DSY296 and C. glabrata DSY565 leading to a reduction of the starting inoculum of 3.1 and 3.0log10CFU, respectively, within 60min of exposure. Under low intensity visible light irradiation, Cu-PES exhibited an accelerated fungicidal activity against both strains with a reduction of 3.0 and 3.4log10CFU, respectively, within 30min of exposure. This effect was likely due to the semiconductor Cu2O/CuO charge separation. The decrease in cell viability of the two Candida strains under dark and light conditions correlated with the progressive loss of membrane integrity. These results indicate that Cu-PES represent a promising strategy for decreasing the colonization of surfaces by yeasts and that actinic light can improve its self-disinfecting activity.

Mots-clé
Antifungal Agents/chemistry, Antifungal Agents/pharmacology, Azoles/pharmacology, Candida albicans/cytology, Candida albicans/drug effects, Candida albicans/radiation effects, Cell Membrane/drug effects, Cell Membrane/metabolism, Cell Membrane/radiation effects, Copper/chemistry, Copper/pharmacology, Darkness, Drug Resistance, Fungal/drug effects, Drug Resistance, Fungal/radiation effects, Polyesters/chemistry, Surface Properties, Actinic light, Antifungal activity, Azole-resistant Candida spp., Copper-sputtered polyester
Pubmed
Web of science
Création de la notice
23/10/2017 9:52
Dernière modification de la notice
20/08/2019 13:23
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