Liquid chromatography-tandem mass spectrometry (LC/APCI-MS/MS) methods for the quantification of captan and folpet phthalimide metabolites in human plasma and urine.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_018075A5937E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Liquid chromatography-tandem mass spectrometry (LC/APCI-MS/MS) methods for the quantification of captan and folpet phthalimide metabolites in human plasma and urine.
Journal
Analytical and Bioanalytical Chemistry
Author(s)
Berthet Aurélie, Bouchard Michèle, Schüpfer Patrick, Vernez David, Danuser Brigitta, Huynh Cong Khanh
ISSN
1618-2642
1618-2650 (Electronic)
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
399
Number
6
Pages
2243-2255
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Captan and folpet are fungicides largely used in agriculture. They have similar chemical structures, except that folpet has an aromatic ring unlike captan. Their half-lives in blood are very short, given that they are readily broken down to tetrahydrophthalimide (THPI) and phthalimide (PI), respectively. Few authors measured these biomarkers in plasma or urine, and analysis was conducted either by gas chromatography coupled to mass spectrometry or liquid chromatography with UV detection. The objective of this study was thus to develop simple, sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (LC/APCI-MS/MS) methods to quantify both THPI and PI in human plasma and urine. Briefly, deuterated THPI was added as an internal standard and purification was performed by solid-phase extraction followed by LC/APCI-MS/MS analysis in negative ion mode for both compounds. Validation of the methods was conducted using spiked blank plasma and urine samples at concentrations ranging from 1 to 250 μg/L and 1 to 50 μg/L, respectively, along with samples of volunteers and workers exposed to captan or folpet. The methods showed a good linearity (R (2) > 0.99), recovery (on average 90% for THPI and 75% for PI), intra- and inter-day precision (RSD, <15%) and accuracy (<20%), and stability. The limit of detection was 0.58 μg/L in urine and 1.47 μg/L in plasma for THPI and 1.14 and 2.17 μg/L, respectively, for PI. The described methods proved to be accurate and suitable to determine the toxicokinetics of both metabolites in human plasma and urine.
Keywords
Captan/blood, Chromatography, Liquid/methods, Humans, Phthalimides/blood, Tandem Mass Spectrometry/methods
Pubmed
Web of science
Open Access
Yes
Create date
10/08/2011 14:59
Last modification date
20/08/2019 12:23
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