Human MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation.

Détails

ID Serval
serval:BIB_016895B6CF25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human MicroRNA Responses Predict Cytomegalovirus Replication Following Solid Organ Transplantation.
Périodique
The Journal of infectious diseases
Auteur(s)
Han S.H., Kumar D., Ferreira V.H., Egli A., Hirsch H.H., Weisser M., Garzoni C., van Delden C., Bochud P.Y., Manuel O., Meylan P., Boggian K., Husain S., Mueller N.J., Humar A.
Collaborateur(s)
Swiss Transplant Cohort Study
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Statut éditorial
Publié
Date de publication
15/02/2017
Peer-reviewed
Oui
Volume
215
Numéro
4
Pages
537-546
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Homo sapiens mature micro-ribonucleic acid (miRNA)-200b-3p and 200c-3p are predicted to bind to 3' untranslated region of mRNA encoding human cytomegalovirus (HCMV) immediate early protein 2 (IE2). We hypothesized that expression of these miRNAs pretransplant could predict HCMV replication after solid organ transplantation (SOT).
A total of 272 SOT recipients were HCMV-seropositive pretransplant and were managed using preemptive therapy. Pretransplant peripheral blood mononuclear cells were stimulated with HCMV followed by collection of RNA 1 day poststimulation. The miRNAs were quantified using real-time reverse transcription-polymerase chain reaction. Human foreskin fibroblasts were transfected with 200b-3p and 200c-3p and infected with HCMV 1 hour post-transfection. Protein was collected at 3 days postinfection (dpi) and 7 dpi underwent immunoblotting for IE2.
Medians of 200b-3p and 200c-3p were significantly lower in recipients with HCMV replication (n = 144) (361.6 vs 552.6, P = .035; 3586.8 vs 12986.8 copies/μL, P = .03, respectively). Multivariate regression revealed that 200b-3p ≥100 copies/μL (odds ratio [OR]: 0.53; P = .02), was associated with less HCMV replication. Transfection with 200b-3p resulted in 2.7- and 2.5-fold decreased IE2 at 3 dpi and 7 dpi, respectively, compared to mock cells.
MicroRNAs may play a biologically relevant role in controlling HCMV replication post-transplant.

Mots-clé
Adult, Aged, Cell Line, Cytomegalovirus/physiology, Cytomegalovirus Infections/virology, Female, Follow-Up Studies, HeLa Cells, Humans, Immediate-Early Proteins/genetics, Immediate-Early Proteins/metabolism, Leukocytes, Mononuclear/metabolism, Leukocytes, Mononuclear/virology, Logistic Models, Male, MicroRNAs/genetics, MicroRNAs/metabolism, Middle Aged, Multivariate Analysis, Organ Transplantation, Prospective Studies, RNA, Messenger/genetics, RNA, Messenger/metabolism, Reproducibility of Results, Virus Replication
Pubmed
Open Access
Oui
Création de la notice
08/01/2017 16:20
Dernière modification de la notice
08/05/2019 13:41
Données d'usage