Modulation of Na,K-ATPase expression during early development of Xenopus laevis

Détails

ID Serval
serval:BIB_00D29571329C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Modulation of Na,K-ATPase expression during early development of Xenopus laevis
Périodique
Developmental Biology
Auteur(s)
Han  Y., Pralong-Zamofing  D., Ackermann  U., Geering  K.
ISSN
0012-1606 (Print)
Statut éditorial
Publié
Date de publication
05/1991
Volume
145
Numéro
1
Pages
174-81
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
In amphibian and mammalian systems, regulation of Na+ transport via the Na,K-ATPase plays an important role in distinct developmental processes such as blastocoele formation and neurulation. In this study, we have followed the Na,K-ATPase activity, the biosynthesis, and the cellular accumulation of catalytic alpha-subunits after fertilization of Xenopus laevis eggs up to neurula formation. Our data show that Na,K-ATPase activity increases significantly between stages 4 and 6 and again between stages 13 and 24. The four-fold rise in Na,K-ATPase activity during blastocoele formation is not mediated by an increased cellular pool of alpha-subunits. On the other hand, a five-fold increase of the biosynthesis rate around midblastula precedes a progressive accumulation up to neurula stage mainly of alpha 1-subunits and to a lesser extent of a second alpha-immunoreactive species. In contrast, newly synthesized glycoproteinic beta 1-subunits of Na,K-ATPase cannot be detected up to late neurula. These data indicate that (1) upregulation of Na,K-ATPase activity during blastocoele and neurula formation are mediated by different regulation mechanisms and (2) alpha- and possibly beta-isoforms are expressed in a developmentally regulated fashion during early Xenopus development.
Mots-clé
Animals Blotting, Western Ca(2+) Mg(2+)-ATPase/metabolism Embryo, Nonmammalian/enzymology/*physiology Female Kinetics Macromolecular Substances Male Microsomes/enzymology Na(+)-K(+)-Exchanging ATPase/analysis/*metabolism Xenopus laevis/*embryology
Pubmed
Web of science
Création de la notice
24/01/2008 13:28
Dernière modification de la notice
03/03/2018 13:12
Données d'usage