Article: article d'un périodique ou d'un magazine.
AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated stress fiber formation.
Journal of Biological Chemistry
Date de publication
Guanine nucleotide exchange factors of the Dbl family relay signals from membrane receptors to Rho family GTPases. We now demonstrate that a longer transcript of the Lbc gene encodes a chimeric molecule, which we have called AKAP-Lbc, that functions as an A-kinase-anchoring protein (AKAP) and a Rho-selective guanine nucleotide exchange factor. Expression of AKAP-Lbc in fibroblasts favors the formation of stress fibers in a Rho-dependent manner. Application of lysophosphatidic acid or selective expression of Galpha(12) enhances cellular AKAP-Lbc activation. Furthermore, biochemical studies indicate that AKAP-Lbc functions as an adaptor protein to selectively couple Galpha(12) to Rho. Thus, AKAP-Lbc anchors PKA and nucleates the assembly of a Rho-mediated signaling pathway.
3T3 Cells, Animals, Cell Line, Cloning, Molecular, Cyclic AMP-Dependent Protein Kinases/metabolism, DNA, Complementary, GTP-Binding Proteins/genetics, GTP-Binding Proteins/metabolism, Humans, Mice, Molecular Sequence Data, Mutagenesis, Protein Binding, Recombinant Fusion Proteins/metabolism
Web of science
Création de la notice
Dernière modification de la notice