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AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated stress fiber formation.
Journal of Biological Chemistry
Guanine nucleotide exchange factors of the Dbl family relay signals from membrane receptors to Rho family GTPases. We now demonstrate that a longer transcript of the Lbc gene encodes a chimeric molecule, which we have called AKAP-Lbc, that functions as an A-kinase-anchoring protein (AKAP) and a Rho-selective guanine nucleotide exchange factor. Expression of AKAP-Lbc in fibroblasts favors the formation of stress fibers in a Rho-dependent manner. Application of lysophosphatidic acid or selective expression of Galpha(12) enhances cellular AKAP-Lbc activation. Furthermore, biochemical studies indicate that AKAP-Lbc functions as an adaptor protein to selectively couple Galpha(12) to Rho. Thus, AKAP-Lbc anchors PKA and nucleates the assembly of a Rho-mediated signaling pathway.
3T3 Cells, Animals, Cell Line, Cloning, Molecular, Cyclic AMP-Dependent Protein Kinases/metabolism, DNA, Complementary, GTP-Binding Proteins/genetics, GTP-Binding Proteins/metabolism, Humans, Mice, Molecular Sequence Data, Mutagenesis, Protein Binding, Recombinant Fusion Proteins/metabolism
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