Random and site-directed mutagenesis of transcriptional regulator proteins implicated in hydrocarbon degradation pathways

Détails

ID Serval
serval:BIB_005831520FFD
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
Random and site-directed mutagenesis of transcriptional regulator proteins implicated in hydrocarbon degradation pathways
Titre du livre
Handbook of hydrocarbon and lipid microbiology
Auteur(s)
Vogne C., Beggah S., van der Meer J. R.
Editeur
Springer
Lieu d'édition
Berlin
ISBN
978-3-540-77584-3
Statut éditorial
Publié
Date de publication
2010
Editeur scientifique
Timmis K. N.
Volume
5
Série
Springer Reference
Numéro de chapitre
83
Pages
4430-4444
Langue
anglais
Notes
Regulatory proteins control to a large extent the expression of metabolic pathways for hydrocarbon degradation. To do so, they have evolved specific protein domains that can interact with chemical effectors, upon which they elicit their influence on the transcriptional process. In order to understand the nature of the recognition domains and the role of specific residues in effector binding, many studies have applied mutagenesis strategies. This chapter will rehearse a few strategies used for mutagenesis of regulatory proteins and screening of effector variants, followed by step-by-step protocols on site-directed and error-prone mutagenesis, and screening of mutant libraries by flow cytometry.
Création de la notice
13/01/2010 14:36
Dernière modification de la notice
03/03/2018 13:10
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