Epithelial sodium channel regulatory proteins identified by functional expression cloning
Détails
ID Serval
serval:BIB_FBBBFA880DFF
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Epithelial sodium channel regulatory proteins identified by functional expression cloning
Périodique
Kidney International. Supplement
ISSN
0098-6577 (Print)
Statut éditorial
Publié
Date de publication
09/1998
Volume
67
Pages
S109-14
Notes
Journal Article
Research Support, Non-U.S. Gov't
Review --- Old month value: Sep
Research Support, Non-U.S. Gov't
Review --- Old month value: Sep
Résumé
We describe here our current strategy for identifying and cloning proteins involved in the regulation of the epithelial sodium channel (ENaC). We have set up a complementation functional assay in the Xenopus laevis oocyte expression system. Using this assay, we have been able to identify a channel-activating protease (CAP-1) that can increase ENaC activity threefold. We propose a novel extracellular signal transduction pathway controlling ionic channels of the ENaC gene family that include genes involved in mechanotransduction (degenerins), in peptide-gated channels involved in neurotransmission (FaNaCh), in proton-gated channels involved in pH sensing (ASIC) or pain sensation (DRASIC).
Mots-clé
Animals
Cloning, Molecular
Epithelial Cells/*chemistry/*physiology
Gene Expression Regulation
Gene Library
Sodium Channels/*genetics/*metabolism
Pubmed
Création de la notice
24/01/2008 13:38
Dernière modification de la notice
20/08/2019 17:26