Epithelial sodium channel regulatory proteins identified by functional expression cloning

Details

Serval ID
serval:BIB_FBBBFA880DFF
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Epithelial sodium channel regulatory proteins identified by functional expression cloning
Journal
Kidney International. Supplement
Author(s)
Vallet  V., Horisberger  J. D., Rossier  B. C.
ISSN
0098-6577 (Print)
Publication state
Published
Issued date
09/1998
Volume
67
Pages
S109-14
Notes
Journal Article
Research Support, Non-U.S. Gov't
Review --- Old month value: Sep
Abstract
We describe here our current strategy for identifying and cloning proteins involved in the regulation of the epithelial sodium channel (ENaC). We have set up a complementation functional assay in the Xenopus laevis oocyte expression system. Using this assay, we have been able to identify a channel-activating protease (CAP-1) that can increase ENaC activity threefold. We propose a novel extracellular signal transduction pathway controlling ionic channels of the ENaC gene family that include genes involved in mechanotransduction (degenerins), in peptide-gated channels involved in neurotransmission (FaNaCh), in proton-gated channels involved in pH sensing (ASIC) or pain sensation (DRASIC).
Keywords
Animals Cloning, Molecular Epithelial Cells/*chemistry/*physiology Gene Expression Regulation Gene Library Sodium Channels/*genetics/*metabolism
Pubmed
Create date
24/01/2008 12:38
Last modification date
20/08/2019 16:26
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