Carcinoembryonic antigen expression, antibody localisation and immunophotodetection of human colon cancer liver metastases in nude mice: a model for radioimmunotherapy.

Détails

ID Serval
serval:BIB_EDED82725C81
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Carcinoembryonic antigen expression, antibody localisation and immunophotodetection of human colon cancer liver metastases in nude mice: a model for radioimmunotherapy.
Périodique
International Journal of Cancer
Auteur(s)
Vogel C.A., Galmiche M.C., Westermann P., Sun L.Q., Pèlegrin A., Folli S., Bischof Delaloye A., Slosman D.O., Mach J.P., Buchegger F.
ISSN
0020-7136 (Print)
ISSN-L
0020-7136
Statut éditorial
Publié
Date de publication
1996
Volume
67
Numéro
2
Pages
294-302
Langue
anglais
Résumé
Colorectal cancer frequently disseminates through the portal vein into the liver. In this study, outbred Swiss nude mice were adapted to facilitate the induction of liver metastases by a pre-grafting treatment with 6 Gy total body irradiation and i.v. injection of anti-asialo GM1 antibody. One day later, cultured LS 174T human colon cancer cells were injected into the surgically exposed spleen, which was resected 3 min later. In 48 of 65 mice, a few to several hundred liver metastases were macroscopically observed at dissection 3 to 4 weeks after transplantation. Ten of 10 mice, followed-up for survival, died with multiple large confluent liver metastases. By reducing the radiation dose to 4 or 0 Gy, or omitting the anti-asialo GM1 antibody injection, only 60%, 37% or 50% of mice, respectively, had visible metastases 3 weeks after transplantation. Carcinoembryonic antigen (CEA) measured in tumour extracts was in the mean 25.6 micrograms/g in liver metastases compared with 9.2 micrograms/g in s.c. tumours. Uptake of radiolabelled anti-CEA monoclonal antibody (MAb) in the metastases 12, 24 and 48 hr after injection gave a mean value of 39% of the injected dose per gram of tissue (ID/g). In comparison, MAb uptake in s.c. and intrasplenic tumours or lung metastases gave a mean percentage ID/g of 20, 18 and 15, respectively. Laser-induced fluorescence after injection of indocyanin-MAb conjugate allowed direct visual detection of small liver metastases, including some that were not visible under normal light. Preliminary results showed that mice, pre-treated with 4 Gy irradiation and the anti-asialo GM1 injection, were tolerant to radioimmunotherapy with a total dose of 500 muCi 131I labeled anti-CEA intact MAbs given in 3 injections.
Mots-clé
Animals, Antibodies/pharmacology, Antibodies, Monoclonal, Carbocyanines, Carcinoembryonic Antigen/analysis, Colonic Neoplasms/immunology, Colonic Neoplasms/pathology, Fluorescence, Fluorescent Dyes, G(M1) Ganglioside/immunology, Humans, Immunohistochemistry, Lasers, Liver Neoplasms/pathology, Liver Neoplasms/radiotherapy, Mice, Mice, Nude, Neoplasm Transplantation, Radioimmunotherapy, Tumor Cells, Cultured, Whole-Body Irradiation
Pubmed
Web of science
Création de la notice
25/01/2008 11:22
Dernière modification de la notice
20/08/2019 16:15
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