Carcinoembryonic antigen expression, antibody localisation and immunophotodetection of human colon cancer liver metastases in nude mice: a model for radioimmunotherapy.

Details

Serval ID
serval:BIB_EDED82725C81
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Carcinoembryonic antigen expression, antibody localisation and immunophotodetection of human colon cancer liver metastases in nude mice: a model for radioimmunotherapy.
Journal
International Journal of Cancer
Author(s)
Vogel C.A., Galmiche M.C., Westermann P., Sun L.Q., Pèlegrin A., Folli S., Bischof Delaloye A., Slosman D.O., Mach J.P., Buchegger F.
ISSN
0020-7136 (Print)
ISSN-L
0020-7136
Publication state
Published
Issued date
1996
Volume
67
Number
2
Pages
294-302
Language
english
Abstract
Colorectal cancer frequently disseminates through the portal vein into the liver. In this study, outbred Swiss nude mice were adapted to facilitate the induction of liver metastases by a pre-grafting treatment with 6 Gy total body irradiation and i.v. injection of anti-asialo GM1 antibody. One day later, cultured LS 174T human colon cancer cells were injected into the surgically exposed spleen, which was resected 3 min later. In 48 of 65 mice, a few to several hundred liver metastases were macroscopically observed at dissection 3 to 4 weeks after transplantation. Ten of 10 mice, followed-up for survival, died with multiple large confluent liver metastases. By reducing the radiation dose to 4 or 0 Gy, or omitting the anti-asialo GM1 antibody injection, only 60%, 37% or 50% of mice, respectively, had visible metastases 3 weeks after transplantation. Carcinoembryonic antigen (CEA) measured in tumour extracts was in the mean 25.6 micrograms/g in liver metastases compared with 9.2 micrograms/g in s.c. tumours. Uptake of radiolabelled anti-CEA monoclonal antibody (MAb) in the metastases 12, 24 and 48 hr after injection gave a mean value of 39% of the injected dose per gram of tissue (ID/g). In comparison, MAb uptake in s.c. and intrasplenic tumours or lung metastases gave a mean percentage ID/g of 20, 18 and 15, respectively. Laser-induced fluorescence after injection of indocyanin-MAb conjugate allowed direct visual detection of small liver metastases, including some that were not visible under normal light. Preliminary results showed that mice, pre-treated with 4 Gy irradiation and the anti-asialo GM1 injection, were tolerant to radioimmunotherapy with a total dose of 500 muCi 131I labeled anti-CEA intact MAbs given in 3 injections.
Keywords
Animals, Antibodies/pharmacology, Antibodies, Monoclonal, Carbocyanines, Carcinoembryonic Antigen/analysis, Colonic Neoplasms/immunology, Colonic Neoplasms/pathology, Fluorescence, Fluorescent Dyes, G(M1) Ganglioside/immunology, Humans, Immunohistochemistry, Lasers, Liver Neoplasms/pathology, Liver Neoplasms/radiotherapy, Mice, Mice, Nude, Neoplasm Transplantation, Radioimmunotherapy, Tumor Cells, Cultured, Whole-Body Irradiation
Pubmed
Web of science
Create date
25/01/2008 12:22
Last modification date
20/08/2019 17:15
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